5-Amino-1MQ

Mechanism, Dosing, Effects, and Safety

5-Amino-1MQ is a selective, cell-permeable NNMT (Nicotinamide N-methyltransferase) inhibitor studied for its potential to enhance metabolic efficiency, support fat oxidation, preserve lean mass, and increase intracellular NAD⁺ availability. Its mechanism is metabolic rather than appetite-suppressive.

Mechanism of Action

NNMT is an enzyme that methylates nicotinamide, reducing its availability for NAD⁺ synthesis. In metabolic dysfunction, NNMT is often overexpressed in adipose tissue.

By inhibiting NNMT, 5-Amino-1MQ may:

Preserve nicotinamide for NAD⁺ regeneration
Activate SIRT1 and mitochondrial biogenesis pathways
Increase cellular energy efficiency
Support fat loss without suppressing appetite

Dosing Strategy

Route: Subcutaneous
Vial: 50 mg

Reconstitution

Add 3.0 mL bacteriostatic water
Concentration: 16.7 mg/mL
U-100 insulin syringe:
1 unit (0.01 mL) ≈ 0.167 mg (167 mcg)

Cycle Protocol

Week 1 (Assessment Phase)

2.5 mg once daily
Injection volume: 15 units (0.15 mL)

Weeks 2–4 (Active Phase)

Choose based on tolerance and research goal:

Option A: 2.5 mg once daily (maintenance)
Option B: 5.0 mg once daily (enhanced effect)

5 mg dose reference:

30 units (0.30 mL)

Cycle Length

On cycle: 4 weeks
Off cycle: 4 weeks
Repeat: After full rest period if desired

Vial usage

2.5 mg/day → ~20 days per vial
5 mg/day → ~10 days per vial

Effects and Outcomes

Observed in preclinical and early research:

Improved fat metabolism
Increased NAD⁺ availability
Activation of SIRT1 pathways
Preservation of lean tissue
No direct appetite suppression

Often stacked with training, calorie control, and sleep optimization for best results.

Safety and Tolerability

Generally well tolerated in research settings.

Common observations

Mild injection-site irritation
Transient headache or stimulation
Mild stinging on injection

Safety notes

Avoid continuous long-term use
Respect full 4-week off cycle
Rotate injection sites
Document dosing and response

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