KissPeptin

Mechanism, Dosing, Effects

Kisspeptin (metastin) is a naturally occurring neuroendocrine peptide that plays a central role in reproductive hormone regulation. It acts upstream in the hypothalamus to stimulate gonadotropin-releasing hormone (GnRH), which then triggers release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This makes kisspeptin a powerful regulator of testosterone, estrogen, ovulation, and fertility signaling.

Mechanism of Action

Kisspeptin binds to the GPR54 receptor on GnRH neurons in the hypothalamus. This interaction:

Triggers pulsatile GnRH release
Stimulates pituitary secretion of LH and FSH
Activates the HPG axis (hypothalamic–pituitary–gonadal axis)
Increases endogenous testosterone and estrogen
Supports spermatogenesis and ovulation

Unlike hCG or direct hormone therapy, kisspeptin works upstream, encouraging the body to produce hormones naturally rather than replacing them.

Dosing Strategy

Route: Subcutaneous
Vial: 10 mg

Reconstitution

Add 3.0 mL bacteriostatic water
Concentration: 3.33 mg/mL
U-100 syringe reference:
- 1 unit (0.01 mL) ≈ 33.3 mcg

Standard Cycle Protocol

Weeks 1–2

100 mcg daily
Injection: 3 units (0.03 mL)

Weeks 3–8 (or up to 12)

200 mcg daily
Injection: 6 units (0.06 mL)

Cycle length:

8–12 weeks
Avoid continuous long-term use to prevent receptor desensitization

Effects and Outcomes

Observed in research:

Increased LH and FSH release
Boost in endogenous testosterone / estrogen
Improved fertility signaling
Restoration of GnRH pulsatility
Support for ovulation and spermatogenesis
Physiological hormone stimulation (no shutdown)

Kisspeptin is often explored for:

Fertility protocols
HPG axis support
Post-suppression recovery
IVF ovulation triggering

Safety and Tolerability

Generally well tolerated

Possible side effects

Mild injection-site irritation
Temporary headache
Flushing or warmth
Mild nausea (rare)

Safety notes

Avoid prolonged continuous use
Rotate injection sites
Do not exceed recommended dosing
Monitor hormone-related symptoms
Not studied for long-term daily use

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